Journal: Cellular & Molecular Biology Letters
Article Title: Gut Lachnospiraceae improves white matter injury-related cognitive decline by increasing L-arginine
doi: 10.1186/s11658-026-00866-3
Figure Lengend Snippet: Effects of L-Arg on oligodendrocyte lineage cells, microglia and BBB. A-D Immunofluorescence images and quantification of OPCs (Olig2⁺PDGFRα⁺ cells) and OLs (Olig2⁺CC1⁺ cells) in the CC of mice ( n = 5). E-H Immunofluorescence images and quantification of proliferative OPCs (EdU⁺PDGFRα⁺ cells) and proliferative OLs (EdU⁺CC1⁺ cells) in the CC of mice ( n = 5). I , K PDGFRα staining and quantification of OPCs in the proliferation phase under CoCl 2 -induced hypoxia in vitro ( n = 3). J , L , M CC1 and MBP staining and quantification of OPCs in the differentiation phase under CoCl 2 -induced hypoxia in vitro ( n = 3). N , O Immunofluorescence images and quantification of anti-inflammatory microglia (Arg-1⁺IBA-1⁺ cells) in the CC of mice ( n = 5). P Representative Western blot image and quantification of Arg-1 expression in primary microglia ( n = 3). Q , R Quantitative analysis of immunofluorescence images for Arg-1 in primary microglia ( n = 3). S Immunofluorescence images of the vascular endothelial marker CD31 and FITC-dextran. T Quantitative analysis of FITC-dextran leakage ( n = 5). U , V Representative Western blot images and quantitative analysis of ZO-1 and Occludin expression in Bend3 cells ( n = 3). Scale bar: 50 μm. * p < 0.05, ** p < 0.01, *** p < 0.001
Article Snippet: Protein samples were isolated and homogenized using RIPA (ThermoFisher Scientific, USA) and protease inhibitors (ThermoFisher Scientific, USA), separated by SDS-PAGE, transferred to PVDF membranes, blocked with 5% skim milk, and incubated with the primary antibodies, including eNOS (CST, 9572, 1:1000), p-eNOS (CST, 9574, 1:1000), iNOS (abcam, ab178945, 1:1000), Arg-1 (CST, 93668, 1:1000), ZO-1 (Proteintech, 21773-1-ap, 1:5000), Occludin (Invitrogen, 331500, 1:2000) and GAPDH (Proteintech, 60004-1-ig, 1:1000) for overnight at 4 °C and then incubated with the secondary antibodies for 2 h at room temperature.
Techniques: Immunofluorescence, Staining, In Vitro, Western Blot, Expressing, Marker
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